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NICAS Colloquium Online

28 April 2022 @ 12:00 pm - 1:00 pm CEST

We are pleased to announce a new, online edition of the NICAS colloquium on Thursday 28 April 2022 from 12.00 to 13.00 hrs. The colloquium will take place online through Microsoft Teams. By registering through the form below, you will receive the meeting link in your email.


Throughout the year, NICAS organises a bi-weekly Colloquium consisting of two short research lectures. It provides researchers with the opportunity to present ideas for, updates on or results of their activities. The NICAS Colloquium allows people to stay informed on a regular basis about the latest developments and results of research and to exchange information and expertise.



The chair of this colloquium will be Fréderique Broers (Rijksmuseum)


The presenters are:


 Iris Groeneveld – TooCOLD – Development of a light exposure cell with real-time UV/Vis monitoring coupled to LC-DAD to study photodegradation



In many areas, studying the mechanism of photodegradation is of high importance. Conventional methods to do so can be rather time consuming and prone to experimental errors. We have developed an integrated and automated system for the study of light-induced degradation. This so-called ‘TooCOLD box’ is coupled online to liquid chromatography (LC) with diode array detection (DAD) for immediate and automated analysis of the composition of the light-exposed samples. A liquid core waveguide (LCW) is used as exposure cell, allowing efficient illumination of the sample over a 12-cm path length. This cell is coupled to a spectrograph allowing in-situ absorbance monitoring of the exposed sample during irradiation. The LCW is gas permeable permitting diffusion of air into the cell during light exposure. Performance tests showed high linearity, sensitivity and repeatability of the in-cell spectroscopic monitoring as well as for the photodegradation experiments.



Iris Groeneveld obtained her masters degree in Analytical Chemistry from the University of Amsterdam and the VU Amsterdam in 2018 after which she started her PhD in the TooCOLD project at the Amsterdam Institute for Molecular and Life Sciences at the Vrije Universiteit Amsterdam. She focusses on photodegradation and spectroscopy under the supervision of Maarten van Bommel, Govert Somsen and Freek Ariese.



 Mimi den Uijl – The TooCOLD project in perspective – comparison of photodegradation techniques and further implementation of the light cell



Studying photodegradation can be very challenging. The new ‘TooCOLD’ light cell (Toolbox for studying the Chemistry Of Light-induced Degradation) can aid in understanding these degradation mechanisms. The degradations performed in this light cell, however, are performed in solution. For the TooCOLD light cell to be implemented in cultural heritage research, it is compared to other photodegradation techniques such as the Xenotest and the Microfading tester. These two techniques are applied to dyes on textiles. To compare on-textile and in-solution degradation, a light box was used to compare the matrix effect of degradation. To show the relevance of this modular light cell, it was implemented in a multidimensional liquid chromatography setup to separate the dye from a mixture prior to degradation and to re-introduce the degraded mixture into a second separation.



Mimi den Uijl obtained her masters degree in Analytical Chemistry from the University of Amsterdam and the VU Amsterdam in 2018 after which she started her PhD in the TooCOLD project at the Van ‘t Hoff Institute for Molecular Sciences at the University of Amsterdam. She focusses on photodegradation and multidimensional liquid chromatography under the supervision of Maarten van Bommel and Peter Schoenmakers.

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28 April 2022
12:00 pm - 1:00 pm CEST
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